Assay Detail
Binding
CHEMBL4621647
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Escherichia coli Stx1 in human HeLa cells assessed as stimulation of protein synthesis by measuring increase in [14C]-leucine incorporation incubated with cells for 3 hrs prior to Stx1 addition and further incubated for overnight and subsequent [14C]-leucine addition measured after 6 hrs by microbeta liquid scintillation counting analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Cell Type | HeLa |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Structure-Activity Relationship Studies of Retro-1 Analogues against Shiga Toxin.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 1 | 5.70 | 5.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL145347 | LASALOCID | 2.0 | 1 | 5.70 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL145347 | LASALOCID | EC50 | = | 2000.0 | nM | 5.70 |