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Assay Detail

CHEMBL4622236

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at TLR7 in human whole blood assessed as inhibition of DOTAP-ssRNA40 induced IFNalpha production preincubated for 30 mins followed by ssRNA40 addition measured after 20 hrs by ELISA
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Toll-like receptor 7 (CHEMBL5936)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Target-Based Identification and Optimization of 5-Indazol-5-yl Pyridones as Toll-like Receptor 7 and 8 Antagonists Using a Biochemical TLR8 Antagonist Competition Assay.
Knoepfel T, Nimsgern P, Jacquier S, Bourrel M, Vangrevelinghe E, Glatthar R, Behnke D, Alper PB, Michellys PY, Deane J, Junt T, Zipfel G, Limonta S, Hawtin S, Andre C, Boulay T, Loetscher P, Faller M, Blank J, Feifel R, Betschart C.
J Med Chem (2020) 63 : 8276 -8295
PubMed 32786235 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.68 8.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4641633 1 8.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4641633 IC50 = 2.1 nM 8.68