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Assay Detail

CHEMBL4622407

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of IDO1 in human whole blood stimulated with IFNgamma/LPS using L-tryptophan/kynurenine as substrate incubated for 15 mins prior to IFNgamma/LPS stimulation and further incubated for 18 hrs followed by isotope labeled L-tryptophan/kynurenine addition on day 2 by LC/MS-MS analysis
11
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Indoleamine 2,3-dioxygenase 1 (CHEMBL4685)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Potent and Orally Available Bicyclo[1.1.1]pentane-Derived Indoleamine-2,3-dioxygenase 1 (IDO1) Inhibitors.
Pu Q, Zhang H, Guo L, Cheng M, Doty AC, Ferguson H, Fradera X, Lesburg CA, McGowan MA, Miller JR, Geda P, Song X, Otte K, Sciammetta N, Solban N, Yu W, Sloman DL, Zhou H, Lammens A, Neumann L, Bennett DJ, Pasternak A, Han Y.
ACS Med Chem Lett (2020) 11 : 1548 -1554
PubMed 32832022 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.90 7.52
IC90 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4638223 1 7.52
CHEMBL4644751 2 7.44
CHEMBL4632992 1 6.92
CHEMBL4648765 1 6.90
CHEMBL4642946 1 6.86
CHEMBL4647172 1 6.85
CHEMBL4639353 1 6.81
CHEMBL4638557 1 6.76
CHEMBL4647108 1 6.56
CHEMBL4641517 1 6.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4638223 IC50 = 30.0 nM 7.52
CHEMBL4644751 IC50 = 36.0 nM 7.44
CHEMBL4632992 IC50 = 121.0 nM 6.92
CHEMBL4648765 IC50 = 125.0 nM 6.90
CHEMBL4642946 IC50 = 137.0 nM 6.86
CHEMBL4647172 IC50 = 140.0 nM 6.85
CHEMBL4639353 IC50 = 154.0 nM 6.81
CHEMBL4638557 IC50 = 174.0 nM 6.76
CHEMBL4647108 IC50 = 276.0 nM 6.56
CHEMBL4641517 IC50 = 443.0 nM 6.35
CHEMBL4644751 IC90 = 912.0 nM -