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Assay Detail

CHEMBL4622607

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal Hex-tagged DYRK1A (127 to 485 residues) expressed in Escherichia coli BL21 (DE3) incubated for 1.5 hrs by TR-FRET assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Mining Public Domain Data to Develop Selective DYRK1A Inhibitors.
Henderson SH, Sorrell F, Bennett J, Hanley MT, Robinson S, Hopkins Navratilova I, Elkins JM, Ward SE.
ACS Med Chem Lett (2020) 11 : 1620 -1626
PubMed 32832032 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 7.37 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL495696 GW778894X 1 8.30
CHEMBL4646866 1 8.15
CHEMBL187081 PYRAZOLOPYRIDAZINE 1 1 7.92
CHEMBL359554 1 7.42
CHEMBL366259 1 7.19
CHEMBL4637319 1 7.12
CHEMBL4636572 1 7.01
CHEMBL359794 1 6.99
CHEMBL4643515 1 6.86
CHEMBL360866 1 6.73

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL495696 GW778894X IC50 = 5.0 nM 8.30
CHEMBL4646866 IC50 = 7.0 nM 8.15
CHEMBL187081 PYRAZOLOPYRIDAZINE 1 IC50 = 12.0 nM 7.92
CHEMBL359554 IC50 = 38.0 nM 7.42
CHEMBL366259 IC50 = 64.0 nM 7.19
CHEMBL4637319 IC50 = 76.0 nM 7.12
CHEMBL4636572 IC50 = 97.0 nM 7.01
CHEMBL359794 IC50 = 103.0 nM 6.99
CHEMBL4643515 IC50 = 137.0 nM 6.86
CHEMBL360866 IC50 = 186.0 nM 6.73