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Assay Detail

CHEMBL4623159

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST fused human VEGFR2 cytoplasmic domain (790 to 1356 (end) residues) expressed in baculovirus expression system using FAM-labeled peptide and ATP as substrate preincubated for 10 mins followed by substrate addition measured after 1 hr by mobility shift assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Vascular endothelial growth factor receptor 2 (CHEMBL279)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural modifications of indolinones bearing a pyrrole moiety and discovery of a multi-kinase inhibitor with potent antitumor activity.
Qin M, Tian Y, Han X, Cao Q, Zheng S, Liu C, Wu X, Liu L, Meng Y, Wang X, Zhang H, Hou Y.
Bioorg Med Chem (2020) 28 : 115486 -115486
PubMed 32305182 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.93 8.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL502835 NINTEDANIB 4.0 1 8.33
CHEMBL4644718 1 8.19
CHEMBL4639712 1 7.85
CHEMBL4079087 1 7.23
CHEMBL4643300 1 7.19
CHEMBL4069394 1 7.08
CHEMBL4634687 1 7.00
CHEMBL4640617 1 6.83
CHEMBL4649259 1 6.64
CHEMBL4641639 1 6.61
CHEMBL4640302 1 6.54
CHEMBL4647154 1 6.33
CHEMBL4642920 1 6.31
CHEMBL4648781 1 6.31
CHEMBL4639539 1 6.23
CHEMBL4638817 1 6.16
CHEMBL4647153 1 -
CHEMBL4641415 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL502835 NINTEDANIB IC50 = 4.7 nM 8.33
CHEMBL4644718 IC50 = 6.5 nM 8.19
CHEMBL4639712 IC50 = 14.0 nM 7.85
CHEMBL4079087 IC50 = 58.3 nM 7.23
CHEMBL4643300 IC50 = 65.0 nM 7.19
CHEMBL4069394 IC50 = 82.3 nM 7.08
CHEMBL4634687 IC50 = 100.8 nM 7.00
CHEMBL4640617 IC50 = 147.6 nM 6.83
CHEMBL4649259 IC50 = 227.7 nM 6.64
CHEMBL4641639 IC50 = 248.0 nM 6.61
CHEMBL4640302 IC50 = 288.0 nM 6.54
CHEMBL4647154 IC50 = 472.0 nM 6.33
CHEMBL4642920 IC50 = 492.0 nM 6.31
CHEMBL4648781 IC50 = 485.0 nM 6.31
CHEMBL4639539 IC50 = 584.0 nM 6.23
CHEMBL4638817 IC50 = 700.0 nM 6.16
CHEMBL4647153 IC50 > 1000.0 nM -
CHEMBL4641415 IC50 > 1000.0 nM -