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Assay Detail

CHEMBL4623163

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST-tagged human recombinant PDGFRalpha D842I mutant expressed in baculovirus-infected Sf9 cells using FAM-labeled peptide and ATP as substrate preincubated for 10 mins followed by substrate addition measured after 1 hr by mobility shift assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Structural modifications of indolinones bearing a pyrrole moiety and discovery of a multi-kinase inhibitor with potent antitumor activity.
Qin M, Tian Y, Han X, Cao Q, Zheng S, Liu C, Wu X, Liu L, Meng Y, Wang X, Zhang H, Hou Y.
Bioorg Med Chem (2020) 28 : 115486 -115486
PubMed 32305182 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 8.44 8.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL502835 NINTEDANIB 4.0 1 8.72
CHEMBL4644718 1 8.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL502835 NINTEDANIB IC50 = 1.9 nM 8.72
CHEMBL4644718 IC50 = 7.0 nM 8.15