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Assay Detail

CHEMBL4623385

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to recombinant human RORgammat transfected in human HEK293T cells incubated for 24 hrs by dual-glo luciferase reporter gene assay
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK-293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nuclear receptor ROR-gamma (CHEMBL1741186)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and optimization of new oxadiazole substituted thiazole RORγt inverse agonists through a bioisosteric amide replacement approach.
Steeneck C, Gege C, Kinzel O, Albers M, Kleymann G, Schlüter T, Schulz A, Xue X, Cummings MD, Fourie AM, Leonard KA, Scott B, Edwards JP, Hoffmann T, Goldberg SD.
Bioorg Med Chem Lett (2020) 30 : 127174 -127174
PubMed 32334912 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 8.04 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4635823 1 10.00
CHEMBL4647899 1 8.80
CHEMBL4128926 1 8.70
CHEMBL4129202 1 8.59
CHEMBL4637144 1 8.49
CHEMBL4642631 1 8.44
CHEMBL4632527 1 8.44
CHEMBL4647734 1 8.37
CHEMBL4642450 1 8.22
CHEMBL4649094 1 8.10
CHEMBL4633899 1 8.10
CHEMBL4637003 1 8.05
CHEMBL4645288 1 7.96
CHEMBL4641138 1 7.89
CHEMBL4648607 1 7.87
CHEMBL4642733 1 7.85
CHEMBL4649210 1 7.82
CHEMBL4632490 1 7.80
CHEMBL4649863 1 7.80
CHEMBL4648849 1 7.68
CHEMBL4642674 1 7.66
CHEMBL4633325 1 7.62
CHEMBL4641039 1 7.60
CHEMBL4634072 1 7.57
CHEMBL4634974 1 7.57
CHEMBL4649427 1 7.48
CHEMBL4644116 1 7.35
CHEMBL4645942 1 7.33

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4635823 IC50 = 0.1 nM 10.00
CHEMBL4647899 IC50 = 1.6 nM 8.80
CHEMBL4128926 IC50 = 2.0 nM 8.70
CHEMBL4129202 IC50 = 2.6 nM 8.59
CHEMBL4637144 IC50 = 3.2 nM 8.49
CHEMBL4642631 IC50 = 3.6 nM 8.44
CHEMBL4632527 IC50 = 3.6 nM 8.44
CHEMBL4647734 IC50 = 4.3 nM 8.37
CHEMBL4642450 IC50 = 6.0 nM 8.22
CHEMBL4649094 IC50 = 8.0 nM 8.10
CHEMBL4633899 IC50 = 8.0 nM 8.10
CHEMBL4637003 IC50 = 9.0 nM 8.05
CHEMBL4645288 IC50 = 11.0 nM 7.96
CHEMBL4641138 IC50 = 12.8 nM 7.89
CHEMBL4648607 IC50 = 13.5 nM 7.87
CHEMBL4642733 IC50 = 14.0 nM 7.85
CHEMBL4649210 IC50 = 15.0 nM 7.82
CHEMBL4632490 IC50 = 16.0 nM 7.80
CHEMBL4649863 IC50 = 15.7 nM 7.80
CHEMBL4648849 IC50 = 21.0 nM 7.68
CHEMBL4642674 IC50 = 22.0 nM 7.66
CHEMBL4633325 IC50 = 24.0 nM 7.62
CHEMBL4641039 IC50 = 25.0 nM 7.60
CHEMBL4634072 IC50 = 27.0 nM 7.57
CHEMBL4634974 IC50 = 27.0 nM 7.57
CHEMBL4649427 IC50 = 33.0 nM 7.48
CHEMBL4644116 IC50 = 45.0 nM 7.35
CHEMBL4645942 IC50 = 47.0 nM 7.33