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Assay Detail

CHEMBL4624682

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FAP in human U87MG cells using Suc-Gly-Pro-AMC as substrate by fluorescence based assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U-87MG ATCC
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prolyl endopeptidase FAP (CHEMBL4683)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A cell-based fluorescent assay for FAP inhibitor discovery.
Zhang B, Liu F, Yang MF, Xu J, Wang Z, Zhang J, Wang R, Yang X.
Bioorg Med Chem Lett (2020) 30 : 127253 -127253
PubMed 32527554 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.68 7.26

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3233842 1 7.26
CHEMBL67279 TALABOSTAT 3.0 1 6.65
CHEMBL4640030 1 6.18
CHEMBL4647532 1 5.82
CHEMBL142703 VILDAGLIPTIN 4.0 1 4.10
CHEMBL1422 SITAGLIPTIN 4.0 1 4.07
CHEMBL376359 ALOGLIPTIN 4.0 1 -
CHEMBL385517 SAXAGLIPTIN ANHYDROUS 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3233842 IC50 = 55.0 nM 7.26
CHEMBL67279 TALABOSTAT IC50 = 224.0 nM 6.65
CHEMBL4640030 IC50 = 663.0 nM 6.18
CHEMBL4647532 IC50 = 1504.0 nM 5.82
CHEMBL142703 VILDAGLIPTIN IC50 = 79760.0 nM 4.10
CHEMBL1422 SITAGLIPTIN IC50 = 85340.0 nM 4.07
CHEMBL376359 ALOGLIPTIN IC50 = 1470000.0 nM -
CHEMBL385517 SAXAGLIPTIN ANHYDROUS IC50 = 1720000.0 nM -