Assay Detail
Binding
CHEMBL4625173
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of murine DNMT3A catalytic domain-mediated DNA methylation using 5'-6FAM-tagged 30-mer duplex DNA (17 AT bp) as substrate preincubated for 30 mins followed by enzyme addition
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
DNA (cytosine-5)-methyltransferase 3A (CHEMBL3108652) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
Novel anticancer drug curaxin CBL0137 impairs DNA methylation by eukaryotic DNA methyltransferase Dnmt3a.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 5.29 | 5.52 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL53463 | DOXORUBICIN | 4.0 | 1 | 5.52 |
| CHEMBL4640631 | — | — | 1 | 5.05 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL53463 | DOXORUBICIN | IC50 | = | 3000.0 | nM | 5.52 |
| CHEMBL4640631 | — | IC50 | = | 9000.0 | nM | 5.05 |