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Assay Detail

CHEMBL4625329

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal GST-tagged PLK1 (1 to 603 residues) expressed in baculovirus expression system using casein as substrate incubated for 45 mins by radiometric assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase PLK1 (CHEMBL3024)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and biological evaluation of novel pteridinone derivatives possessing a hydrazone moiety as potent PLK1 inhibitors.
Li Z, Xu L, Zhu L, Zhao Y, Hu T, Yin B, Liu Y, Hou Y.
Bioorg Med Chem Lett (2020) 30 : 127329 -127329
PubMed 32631534 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 9.07 9.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL513909 BI-2536 2.0 1 9.08
CHEMBL1233528 VOLASERTIB 3.0 1 9.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL513909 BI-2536 IC50 = 0.83 nM 9.08
CHEMBL1233528 VOLASERTIB IC50 = 0.87 nM 9.06