Assay Detail
Functional
CHEMBL4625531
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antagonist activity at human recombinant 5-HT7 receptor expressed in CHOK1 Hunter cell preincubated for 30 mins followed by incubation with agonist for 30 to 60 mins by hit-hunter cAMP assay based chemiluminescence analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | CHO-K1 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Structural manipulation of aporphines via C10 nitrogenation leads to the identification of new 5-HT<sub>7A</sub>R ligands.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 6.66 | 6.90 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4636148 | — | — | 1 | 6.90 |
| CHEMBL4633786 | — | — | 1 | 6.58 |
| CHEMBL1256875 | SPIPERONE HYDROCHLORIDE | — | 1 | 6.51 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4636148 | — | IC50 | = | 125.0 | nM | 6.90 |
| CHEMBL4633786 | — | IC50 | = | 260.0 | nM | 6.58 |
| CHEMBL1256875 | SPIPERONE HYDROCHLORIDE | IC50 | = | 310.0 | nM | 6.51 |