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Assay Detail

CHEMBL4625699

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against human HeLa cells assessed as reduction in cell viability preincubated for 12 hrs followed by 4 Gy irradiation and measured after 60 hrs by MTT assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HeLa
Confidence 1 — Organism
Curated By Autocuration

Target

HeLa (CHEMBL399)
Type CELL-LINE
Organism Homo sapiens

Publication

Designing Dihydrofolate Reductase Inhibitors as X-ray Radiosensitizers to Reverse Radioresistance of Cervical Cancer.
Liang Y, Zeng D, You Y, Ma B, Li X, Chen T.
ACS Med Chem Lett (2020) 11 : 1421 -1428
PubMed 32676149 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.47 6.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4641198 1 6.19
CHEMBL34259 METHOTREXATE 4.0 1 6.09
CHEMBL4647373 1 5.64
CHEMBL4635593 1 5.20
CHEMBL4644558 1 4.84
CHEMBL48296 1 4.83
CHEMBL4639534 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4641198 IC50 = 640.0 nM 6.19
CHEMBL34259 METHOTREXATE IC50 = 820.0 nM 6.09
CHEMBL4647373 IC50 = 2300.0 nM 5.64
CHEMBL4635593 IC50 = 6300.0 nM 5.20
CHEMBL4644558 IC50 = 14600.0 nM 4.84
CHEMBL48296 IC50 = 14800.0 nM 4.83
CHEMBL4639534 IC50 > 80000.0 nM -