Assay Detail
Binding
CHEMBL4625702
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human DHFR using dihydrofolate as substrate in presence of NADPH
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Designing Dihydrofolate Reductase Inhibitors as X-ray Radiosensitizers to Reverse Radioresistance of Cervical Cancer.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 7 | 5.68 | 7.08 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.08 |
| CHEMBL4641198 | — | — | 1 | 6.09 |
| CHEMBL4647373 | — | — | 1 | 5.84 |
| CHEMBL4635593 | — | — | 1 | 5.24 |
| CHEMBL4644558 | — | — | 1 | 4.98 |
| CHEMBL48296 | — | — | 1 | 4.87 |
| CHEMBL4639534 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 83.0 | nM | 7.08 |
| CHEMBL4641198 | — | IC50 | = | 810.0 | nM | 6.09 |
| CHEMBL4647373 | — | IC50 | = | 1430.0 | nM | 5.84 |
| CHEMBL4635593 | — | IC50 | = | 5800.0 | nM | 5.24 |
| CHEMBL4644558 | — | IC50 | = | 10400.0 | nM | 4.98 |
| CHEMBL48296 | — | IC50 | = | 13600.0 | nM | 4.87 |
| CHEMBL4639534 | — | IC50 | > | 50000.0 | nM | - |