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Assay Detail

CHEMBL4627009

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as inhibition of 5HT-induced intracellular Ca2+ mobilization incubated for 1 hr by Fluo-4 dye based fluorescence assay
34
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2A (CHEMBL224)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Pharmacological Characterization of Heterobivalent Ligands for the Putative 5-HT<sub>2A</sub>/mGlu<sub>2</sub> Receptor Complex.
Poulie CBM, Liu N, Jensen AA, Bunch L.
J Med Chem (2020) 63 : 9928 -9949
PubMed 32815361 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 34 6.51 9.09

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4638006 2 9.09
CHEMBL4636980 2 8.03
CHEMBL489624 2 7.96
CHEMBL4638687 2 6.57
CHEMBL4639821 2 6.55
CHEMBL4641860 2 6.52
CHEMBL4645193 2 6.39
CHEMBL4635373 2 6.35
CHEMBL4635960 2 6.29
CHEMBL4646555 2 6.14
CHEMBL4642761 2 5.92
CHEMBL2206440 2 5.89
CHEMBL4643961 2 5.41
CHEMBL4646835 2 5.39
CHEMBL4635729 2 5.22
CHEMBL4645305 2 -
CHEMBL4632373 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4638006 IC50 = 0.8128 nM 9.09
CHEMBL4638006 IC50 = 0.81 nM 9.09
CHEMBL4636980 IC50 = 9.3 nM 8.03
CHEMBL4636980 IC50 = 9.333 nM 8.03
CHEMBL489624 IC50 = 10.96 nM 7.96
CHEMBL489624 IC50 = 11.0 nM 7.96
CHEMBL4638687 IC50 = 270.0 nM 6.57
CHEMBL4638687 IC50 = 275.42 nM 6.56
CHEMBL4639821 IC50 = 280.0 nM 6.55
CHEMBL4639821 IC50 = 288.4 nM 6.54
CHEMBL4641860 IC50 = 302.0 nM 6.52
CHEMBL4641860 IC50 = 300.0 nM 6.52
CHEMBL4645193 IC50 = 410.0 nM 6.39
CHEMBL4645193 IC50 = 407.38 nM 6.39
CHEMBL4635373 IC50 = 446.68 nM 6.35
CHEMBL4635373 IC50 = 450.0 nM 6.35
CHEMBL4635960 IC50 = 512.86 nM 6.29
CHEMBL4635960 IC50 = 520.0 nM 6.28
CHEMBL4646555 IC50 = 720.0 nM 6.14
CHEMBL4646555 IC50 = 724.44 nM 6.14
CHEMBL4642761 IC50 = 1200.0 nM 5.92
CHEMBL4642761 IC50 = 1202.26 nM 5.92
CHEMBL2206440 IC50 = 1288.25 nM 5.89
CHEMBL2206440 IC50 = 1300.0 nM 5.89
CHEMBL4643961 IC50 = 3900.0 nM 5.41
CHEMBL4643961 IC50 = 3981.07 nM 5.40
CHEMBL4646835 IC50 = 4100.0 nM 5.39
CHEMBL4646835 IC50 = 4168.69 nM 5.38
CHEMBL4635729 IC50 = 6025.6 nM 5.22
CHEMBL4635729 IC50 = 6000.0 nM 5.22
CHEMBL4645305 IC50 > 50118.72 nM -
CHEMBL4632373 IC50 > 50118.72 nM -
CHEMBL4632373 IC50 > 50000.0 nM -
CHEMBL4645305 IC50 > 50000.0 nM -