Assay Detail
Binding
CHEMBL4627976
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Inhibition of NS5B polymerase in HCV genotype 1a H77 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Hepatitis C virus subtype 1a |
| Cell Type | Huh-7 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
RNA-directed RNA polymerase (CHEMBL4296320) ↗| Type | SINGLE PROTEIN |
| Organism | Hepacivirus hominis |
Publication
Discovery of 2-aminoisobutyric acid ethyl ester (AIBEE) phosphoramidate prodrugs for delivering nucleoside HCV NS5B polymerase inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 15 | 6.47 | 7.03 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4635092 | — | — | 1 | 7.03 |
| CHEMBL4647104 | — | — | 1 | 7.01 |
| CHEMBL4641335 | — | — | 1 | 6.97 |
| CHEMBL4636436 | — | — | 1 | 6.94 |
| CHEMBL1259059 | SOFOSBUVIR | 4.0 | 1 | 6.81 |
| CHEMBL4639827 | — | — | 1 | 6.81 |
| CHEMBL4644488 | — | — | 1 | 6.53 |
| CHEMBL4633686 | — | — | 1 | 6.50 |
| CHEMBL4639671 | — | — | 1 | 6.38 |
| CHEMBL4642954 | — | — | 1 | 6.38 |
| CHEMBL4644867 | — | — | 1 | 6.23 |
| CHEMBL4635910 | — | — | 1 | 6.02 |
| CHEMBL4634173 | — | — | 1 | 5.96 |
| CHEMBL4637964 | — | — | 1 | 5.92 |
| CHEMBL4640222 | — | — | 1 | 5.60 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4635092 | — | EC50 | = | 94.0 | nM | 7.03 |
| CHEMBL4647104 | — | EC50 | = | 98.0 | nM | 7.01 |
| CHEMBL4641335 | — | EC50 | = | 106.0 | nM | 6.97 |
| CHEMBL4636436 | — | EC50 | = | 114.0 | nM | 6.94 |
| CHEMBL1259059 | SOFOSBUVIR | EC50 | = | 155.0 | nM | 6.81 |
| CHEMBL4639827 | — | EC50 | = | 156.0 | nM | 6.81 |
| CHEMBL4644488 | — | EC50 | = | 298.0 | nM | 6.53 |
| CHEMBL4633686 | — | EC50 | = | 317.0 | nM | 6.50 |
| CHEMBL4639671 | — | EC50 | = | 415.0 | nM | 6.38 |
| CHEMBL4642954 | — | EC50 | = | 414.0 | nM | 6.38 |
| CHEMBL4644867 | — | EC50 | = | 592.0 | nM | 6.23 |
| CHEMBL4635910 | — | EC50 | = | 960.0 | nM | 6.02 |
| CHEMBL4634173 | — | EC50 | = | 1090.0 | nM | 5.96 |
| CHEMBL4637964 | — | EC50 | = | 1210.0 | nM | 5.92 |
| CHEMBL4640222 | — | EC50 | = | 2500.0 | nM | 5.60 |