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Assay Detail

CHEMBL4629361

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Reversible inhibition of human MAGL L179S/L186S mutant using 4-methylumbelliferyl butyrate as substrate measured after 5 mins by fluorescence assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

The discovery of diazetidinyl diamides as potent and reversible inhibitors of monoacylglycerol lipase (MAGL).
Zhu B, Connolly PJ, Zhang SP, Chevalier KM, Milligan CM, Flores CM, Macielag MJ.
Bioorg Med Chem Lett (2020) 30 : 127198 -127198
PubMed 32334914 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.47 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4647633 1 8.22
CHEMBL4643128 1 8.15
CHEMBL1615314 1 8.00
CHEMBL4642828 1 7.82
CHEMBL4642478 1 7.82
CHEMBL4645891 1 7.82
CHEMBL4645008 1 7.75
CHEMBL4639530 1 7.72
CHEMBL4649797 1 7.55
CHEMBL4641944 1 7.51
CHEMBL4633650 1 7.32
CHEMBL4646726 1 6.57
CHEMBL4638423 1 6.34
CHEMBL4633969 1 6.03
CHEMBL4647248 1 -
CHEMBL4649126 1 -
CHEMBL4637281 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4647633 IC50 = 6.0 nM 8.22
CHEMBL4643128 IC50 = 7.0 nM 8.15
CHEMBL1615314 IC50 = 10.0 nM 8.00
CHEMBL4642828 IC50 = 15.0 nM 7.82
CHEMBL4642478 IC50 = 15.0 nM 7.82
CHEMBL4645891 IC50 = 15.0 nM 7.82
CHEMBL4645008 IC50 = 18.0 nM 7.75
CHEMBL4639530 IC50 = 19.0 nM 7.72
CHEMBL4649797 IC50 = 28.0 nM 7.55
CHEMBL4641944 IC50 = 31.0 nM 7.51
CHEMBL4633650 IC50 = 48.0 nM 7.32
CHEMBL4646726 IC50 = 270.0 nM 6.57
CHEMBL4638423 IC50 = 458.0 nM 6.34
CHEMBL4633969 IC50 = 939.0 nM 6.03
CHEMBL4647248 IC50 < 5.0 nM -
CHEMBL4649126 IC50 < 5.0 nM -
CHEMBL4637281 IC50 < 5.0 nM -