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Assay Detail

CHEMBL4630174

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant N-terminal His-tagged LSD1 (unknown origin) expressed in Escherichia coli expression system using ART-K(Me1)-QTARKSTGGKAPRKQLAGGK(Biotin) as substrate incubated for 60 mins by TR-FRET assay
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific histone demethylase 1A (CHEMBL6136)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Synthesis of Styrenylcyclopropylamine LSD1 Inhibitors.
Gehling VS, McGrath JP, Duplessis M, Khanna A, Brucelle F, Vaswani RG, Côté A, Stuckey J, Watson V, Cummings RT, Balasubramanian S, Iyer P, Sawant P, Good AC, Albrecht BK, Harmange JC, Audia JE, Bellon SF, Trojer P, Levell JR.
ACS Med Chem Lett (2020) 11 : 1213 -1220
PubMed 32551003 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 7.12 8.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4646795 1 8.59
CHEMBL4644907 1 8.54
CHEMBL4637451 1 8.52
CHEMBL4636174 1 8.40
CHEMBL4640768 1 8.38
CHEMBL4647030 1 8.28
CHEMBL4642534 1 8.14
CHEMBL4641803 1 8.11
CHEMBL4638408 1 8.06
CHEMBL4645640 1 7.77
CHEMBL4639957 1 7.70
CHEMBL4644039 1 7.60
CHEMBL4647263 1 7.55
CHEMBL4649322 1 7.25
CHEMBL4645912 1 6.70
CHEMBL3786182 GSK2879552 1.0 1 6.67
CHEMBL4640829 1 6.38
CHEMBL4632371 1 6.35
CHEMBL4637228 1 6.10
CHEMBL4646049 1 6.05
CHEMBL4643691 1 5.37
CHEMBL4639078 1 5.03
CHEMBL4634330 1 4.69
CHEMBL3989843 TRANYLCYPROMINE 4.0 1 4.64
CHEMBL4646146 1 -
CHEMBL4649041 1 -
CHEMBL4646431 1 -
CHEMBL4638015 1 -
CHEMBL4637061 1 -
CHEMBL4637906 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4646795 IC50 = 2.6 nM 8.59
CHEMBL4644907 IC50 = 2.9 nM 8.54
CHEMBL4637451 IC50 = 3.0 nM 8.52
CHEMBL4636174 IC50 = 4.0 nM 8.40
CHEMBL4640768 IC50 = 4.2 nM 8.38
CHEMBL4647030 IC50 = 5.3 nM 8.28
CHEMBL4642534 IC50 = 7.2 nM 8.14
CHEMBL4641803 IC50 = 7.7 nM 8.11
CHEMBL4638408 IC50 = 8.8 nM 8.06
CHEMBL4645640 IC50 = 17.0 nM 7.77
CHEMBL4639957 IC50 = 20.0 nM 7.70
CHEMBL4644039 IC50 = 25.0 nM 7.60
CHEMBL4647263 IC50 = 28.0 nM 7.55
CHEMBL4649322 IC50 = 56.0 nM 7.25
CHEMBL4645912 IC50 = 200.0 nM 6.70
CHEMBL3786182 GSK2879552 IC50 = 212.0 nM 6.67
CHEMBL4640829 IC50 = 412.0 nM 6.38
CHEMBL4632371 IC50 = 444.0 nM 6.35
CHEMBL4637228 IC50 = 788.0 nM 6.10
CHEMBL4646049 IC50 = 898.0 nM 6.05
CHEMBL4643691 IC50 = 4280.0 nM 5.37
CHEMBL4639078 IC50 = 9400.0 nM 5.03
CHEMBL4634330 IC50 = 20400.0 nM 4.69
CHEMBL3989843 TRANYLCYPROMINE IC50 = 22900.0 nM 4.64
CHEMBL4646146 IC50 < 2.8 nM -
CHEMBL4649041 IC50 < 3.0 nM -
CHEMBL4646431 IC50 < 3.0 nM -
CHEMBL4638015 IC50 < 3.0 nM -
CHEMBL4637061 IC50 < 2.6 nM -
CHEMBL4637906 IC50 < 1.8 nM -