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Assay Detail

CHEMBL4668809

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 48 hrs by SRB assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type MDA-MB-231
Confidence 1 — Organism
Curated By Autocuration

Target

MDA-MB-231 (CHEMBL400)
Type CELL-LINE
Organism Homo sapiens

Publication

Triple-Negative Breast Cancer Cells Exhibit Differential Sensitivity to Cardenolides from Calotropis gigantea.
Pederson PJ,Cai S,Carver C,Powell DR,Risinger AL,Grkovic T,O'Keefe BR,Mooberry SL,Cichewicz RH
J Nat Prod (2020) 83 : 2269 -2280
PubMed 32649211 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 5.83 6.99

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL500585 USCHARIN 1 6.99
CHEMBL495949 CALOTROPIN 1 6.36
CHEMBL1751 DIGOXIN 4.0 1 6.32
CHEMBL4743046 1 6.20
CHEMBL4797949 1 6.01
CHEMBL4781168 1 5.90
CHEMBL449767 DESGLUCOUZARIN 1 5.00
CHEMBL4776690 1 5.00
CHEMBL495179 COROGLAUCIGENIN 1 4.70
CHEMBL109863 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL500585 USCHARIN IC50 = 102.0 nM 6.99
CHEMBL495949 CALOTROPIN IC50 = 440.0 nM 6.36
CHEMBL1751 DIGOXIN IC50 = 480.0 nM 6.32
CHEMBL4743046 IC50 = 630.0 nM 6.20
CHEMBL4797949 IC50 = 980.0 nM 6.01
CHEMBL4781168 IC50 = 1250.0 nM 5.90
CHEMBL449767 DESGLUCOUZARIN IC50 = 10000.0 nM 5.00
CHEMBL4776690 IC50 = 10000.0 nM 5.00
CHEMBL495179 COROGLAUCIGENIN IC50 = 20000.0 nM 4.70
CHEMBL109863 IC50 > 30000.0 nM -