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Assay Detail

CHEMBL4669201

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of RPE65 in bovine RPE microsomes assessed as reduction in 11-cis-retinol production using all-trans-retinol as substrate pre-incubated with enzyme for 5 mins followed by further incubation with all-trans-retinol for 1 hr by normal phase HPLC analysis
4
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Tissue Retina
Subcellular Microsome
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Retinoid isomerohydrolase (CHEMBL4523306)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Development of chiral fluorinated alkyl derivatives of emixustat as drug candidates for the treatment of retinal degenerative diseases.
Blum E,Zhang J,Korshin E,Palczewski K,Gruzman A
Bioorg Med Chem Lett (2020) 30 : 127421 -127421
PubMed 32717613 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 6.69 6.78

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4795212 2 6.78
CHEMBL4781327 1 6.76
CHEMBL4757869 1 6.49

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4795212 EC50 = 165.6 nM 6.78
CHEMBL4781327 EC50 = 172.4 nM 6.76
CHEMBL4795212 EC50 = 196.5 nM 6.71
CHEMBL4757869 EC50 = 323.3 nM 6.49