Assay Detail
Binding
CHEMBL4669268
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant His6x-tagged human SphK1 expressed in HEK293 cells assessed as reduction in S1P formation using D-erythro-sphingosine as substrate by Western blot analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Development of SKI-349, a dual-targeted inhibitor of sphingosine kinase and microtubule polymerization.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 5.02 | 5.52 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1289102 | — | — | 1 | 5.52 |
| CHEMBL552462 | — | — | 1 | 4.52 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1289102 | — | IC50 | = | 3000.0 | nM | 5.52 |
| CHEMBL552462 | — | IC50 | = | 30000.0 | nM | 4.52 |