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Assay Detail

CHEMBL4669456

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at recombinant hexa-His-tagged THRalpha LBD (202 to 461 residues) (unknown origin)/recombinant human EE-tagged RXRalpha LBD (225 to 462 residues) assessed as increase in biotinylated GRIP1 peptide recruitment preincubated for 30 mins followed by peptide addition and measured after 30 mins by HTRF assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Selective thyroid hormone receptor β agonists with oxadiazolone acid isosteres.
Kirschberg TA,Jones CT,Xu Y,Fenaux M,Halcomb RL,Wang Y,Klucher K
Bioorg Med Chem Lett (2020) 30 : 127465 -127465
PubMed 32768645 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 18 5.76 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL471488 MB07344 -1.0 1 7.89
CHEMBL1544 LIOTHYRONINE 4.0 1 7.52
CHEMBL4792904 1 6.89
CHEMBL4750520 1 6.77
CHEMBL4758498 1 6.47
CHEMBL4789231 1 6.30
CHEMBL4782668 1 5.85
CHEMBL4743629 1 5.75
CHEMBL3261331 RESMETIROM 4.0 1 5.52
CHEMBL4787796 1 5.39
CHEMBL4792525 1 5.19
CHEMBL4776645 1 5.09
CHEMBL4745568 1 5.03
CHEMBL4755097 1 4.95
CHEMBL4759495 1 4.90
CHEMBL4741763 1 4.88
CHEMBL4790442 1 4.79
CHEMBL4753226 1 4.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL471488 MB07344 EC50 = 13.0 nM 7.89
CHEMBL1544 LIOTHYRONINE EC50 = 30.0 nM 7.52
CHEMBL4792904 EC50 = 130.0 nM 6.89
CHEMBL4750520 EC50 = 170.0 nM 6.77
CHEMBL4758498 EC50 = 340.0 nM 6.47
CHEMBL4789231 EC50 = 500.0 nM 6.30
CHEMBL4782668 EC50 = 1400.0 nM 5.85
CHEMBL4743629 EC50 = 1800.0 nM 5.75
CHEMBL3261331 RESMETIROM EC50 = 3000.0 nM 5.52
CHEMBL4787796 EC50 = 4100.0 nM 5.39
CHEMBL4792525 EC50 = 6500.0 nM 5.19
CHEMBL4776645 EC50 = 8200.0 nM 5.09
CHEMBL4745568 EC50 = 9300.0 nM 5.03
CHEMBL4755097 EC50 = 11300.0 nM 4.95
CHEMBL4759495 EC50 = 12600.0 nM 4.90
CHEMBL4741763 EC50 = 13300.0 nM 4.88
CHEMBL4790442 EC50 = 16400.0 nM 4.79
CHEMBL4753226 EC50 = 31300.0 nM 4.50