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Assay Detail

CHEMBL4670936

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal His6-tagged HER2-A775_G776insYVMA mutant (703 to 1029 residues) (unknown origin) expressed in Sf9 insect cells using TK as substrate measured up to 2 to 40 mins by fluorescence assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Targeting Her2-insYVMA with Covalent Inhibitors-A Focused Compound Screening and Structure-Based Design Approach.
Lategahn J,Hardick J,Grabe T,Niggenaber J,Jeyakumar K,Keul M,Tumbrink HL,Becker C,Hodson L,Kirschner T,Klövekorn P,Ketzer J,Baumann M,Terheyden S,Unger A,Weisner J,Müller MP,van Otterlo WAL,Bauer S,Rauh D
J Med Chem (2020) 63 : 11725 -11755
PubMed 32931277 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 7.65 9.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1173655 AFATINIB 4.0 1 9.12
CHEMBL180022 NERATINIB 4.0 1 8.20
CHEMBL4757145 1 7.31
CHEMBL4787483 1 7.27
CHEMBL4756368 1 7.17
CHEMBL4795165 1 6.84

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1173655 AFATINIB Ki = 0.76 nM 9.12
CHEMBL180022 NERATINIB Ki = 6.3 nM 8.20
CHEMBL4757145 Ki = 49.0 nM 7.31
CHEMBL4787483 Ki = 54.0 nM 7.27
CHEMBL4756368 Ki = 67.0 nM 7.17
CHEMBL4795165 Ki = 143.0 nM 6.84