Assay Detail
Binding
CHEMBL4671306
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human RANKL-induced osteoclastogenesis in RANKL cultured mouse bone marrow cells assessed as reduction in RANKL/M-CSF-induced TRAP activity preincubated with RANKL for 1 hr followed by cell addition and subsequent media replenishment every 48 hrs for 4 days
16
Total Activities
11
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Bone marrow cell |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Tumor necrosis factor ligand superfamily member 11 (CHEMBL2364162) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Discovery of Small-Molecule Inhibitors of Receptor Activator of Nuclear Factor-κB Ligand with a Superior Therapeutic Index.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 11 | 5.59 | 6.00 |
| Inhibition | 5 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL255489 | — | — | 2 | 6.00 |
| CHEMBL4760595 | — | — | 1 | 5.85 |
| CHEMBL3742191 | — | — | 2 | 5.73 |
| CHEMBL4797434 | — | — | 1 | 5.72 |
| CHEMBL4750116 | — | — | 1 | 5.70 |
| CHEMBL4756975 | — | — | 2 | 5.60 |
| CHEMBL4745590 | — | — | 1 | 5.57 |
| CHEMBL3741720 | — | — | 2 | 5.54 |
| CHEMBL3741275 | — | — | 2 | 5.29 |
| CHEMBL4752672 | — | — | 1 | 5.29 |
| CHEMBL4745373 | — | — | 1 | 5.19 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL255489 | — | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL4760595 | — | IC50 | = | 1400.0 | nM | 5.85 |
| CHEMBL3742191 | — | IC50 | = | 1880.0 | nM | 5.73 |
| CHEMBL4797434 | — | IC50 | = | 1900.0 | nM | 5.72 |
| CHEMBL4750116 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL4756975 | — | IC50 | = | 2500.0 | nM | 5.60 |
| CHEMBL4745590 | — | IC50 | = | 2700.0 | nM | 5.57 |
| CHEMBL3741720 | — | IC50 | = | 2860.0 | nM | 5.54 |
| CHEMBL3741275 | — | IC50 | = | 5070.0 | nM | 5.29 |
| CHEMBL4752672 | — | IC50 | = | 5100.0 | nM | 5.29 |
| CHEMBL4745373 | — | IC50 | = | 6400.0 | nM | 5.19 |
| CHEMBL3742191 | — | Inhibition | - | % | - | |
| CHEMBL255489 | — | Inhibition | - | % | - | |
| CHEMBL3741720 | — | Inhibition | - | % | - | |
| CHEMBL3741275 | — | Inhibition | - | % | - | |
| CHEMBL4756975 | — | Inhibition | - | % | - |