Assay Detail
Binding
CHEMBL4672060
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to recombinant biotinylated human MIF expressed in Escherichia coli BL21 DE3 by surface plasmon resonance assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Macrophage migration inhibitory factor (CHEMBL2085) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Inhibition of Macrophage Migration Inhibitory Factor by a Chimera of Two Allosteric Binders.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 3 | 5.35 | 5.85 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL19449 | IBUDILAST | 4.0 | 1 | 5.85 |
| CHEMBL4785454 | — | — | 1 | 5.16 |
| CHEMBL296966 | CHICAGO SKY BLUE SODIUM | — | 1 | 5.04 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL19449 | IBUDILAST | Kd | = | 1400.0 | nM | 5.85 |
| CHEMBL4785454 | — | Kd | = | 6860.0 | nM | 5.16 |
| CHEMBL296966 | CHICAGO SKY BLUE SODIUM | Kd | = | 9040.0 | nM | 5.04 |