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Assay Detail

CHEMBL4672956

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant full length wild-type CDK2/Cyclin E1 (unknown origin) expressed in baculovirus expression system assessed as reduction in production of ADP using 5-FAM-QSPKKG-CONH2 peptide as substrate preincubated with enzyme for 15 mins followed by further incubation with ATP for 45 mins by fluorescence-based microfluidic mobility shift assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Cyclin-dependent kinase 2/cyclin E1 (CHEMBL1907605)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Novel CDK2 Inhibitors for Treating Cancer.
Sabnis RW
ACS Med Chem Lett (2020) 11 : 2346 -2347
PubMed 33335646 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 9.66 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4744945 1 10.00
CHEMBL4764153 1 9.92
CHEMBL4758192 1 9.82
CHEMBL4758595 1 9.77
CHEMBL4744114 1 9.34
CHEMBL4763408 1 9.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4744945 Ki = 0.1 nM 10.00
CHEMBL4764153 Ki = 0.12 nM 9.92
CHEMBL4758192 Ki = 0.15 nM 9.82
CHEMBL4758595 Ki = 0.17 nM 9.77
CHEMBL4744114 Ki = 0.46 nM 9.34
CHEMBL4763408 Ki = 0.77 nM 9.11