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Assay Detail

CHEMBL4673080

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PI3Kdelta in human Ramos cells assessed as reduction in anti-IgM-induced AKT phosphorylation at Ser473 residue preincubated for 20 mins followed by anti-IgM stimulation for 30 mins
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Ramos
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Optimization of Versatile Oxindoles as Selective PI3Kδ Inhibitors.
Methot JL,Achab A,Christopher M,Zhou H,McGowan MA,Trotter BW,Fradera X,Lesburg CA,Goldenblatt P,Hill A,Chen D,Otte KM,Augustin M,Shah S,Katz JD
ACS Med Chem Lett (2020) 11 : 2461 -2469
PubMed 33335668 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 6.93 7.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4792586 1 7.64
CHEMBL4795757 1 7.58
CHEMBL4754014 1 7.52
CHEMBL4787672 1 7.50
CHEMBL4452905 1 7.32
CHEMBL4757468 1 7.26
CHEMBL4753205 1 7.16
CHEMBL4776201 1 7.15
CHEMBL4743715 1 7.14
CHEMBL4762936 1 7.07
CHEMBL4781592 1 7.00
CHEMBL4748947 1 6.96
CHEMBL4761378 1 6.96
CHEMBL4744546 1 6.92
CHEMBL4779781 1 6.89
CHEMBL4745502 1 6.75
CHEMBL4745624 1 6.72
CHEMBL4778656 1 6.72
CHEMBL4743750 1 6.70
CHEMBL4750385 1 6.70
CHEMBL4745746 1 6.70
CHEMBL4750236 1 6.64
CHEMBL4791004 1 6.60
CHEMBL4800086 1 6.50
CHEMBL4781485 1 6.36
CHEMBL4800583 1 6.35
CHEMBL4744508 1 6.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4792586 IC50 = 23.0 nM 7.64
CHEMBL4795757 IC50 = 26.0 nM 7.58
CHEMBL4754014 IC50 = 30.0 nM 7.52
CHEMBL4787672 IC50 = 32.0 nM 7.50
CHEMBL4452905 IC50 = 48.0 nM 7.32
CHEMBL4757468 IC50 = 55.0 nM 7.26
CHEMBL4753205 IC50 = 70.0 nM 7.16
CHEMBL4776201 IC50 = 71.0 nM 7.15
CHEMBL4743715 IC50 = 72.0 nM 7.14
CHEMBL4762936 IC50 = 85.0 nM 7.07
CHEMBL4781592 IC50 = 100.0 nM 7.00
CHEMBL4748947 IC50 = 110.0 nM 6.96
CHEMBL4761378 IC50 = 110.0 nM 6.96
CHEMBL4744546 IC50 = 120.0 nM 6.92
CHEMBL4779781 IC50 = 130.0 nM 6.89
CHEMBL4745502 IC50 = 180.0 nM 6.75
CHEMBL4745624 IC50 = 190.0 nM 6.72
CHEMBL4778656 IC50 = 190.0 nM 6.72
CHEMBL4743750 IC50 = 200.0 nM 6.70
CHEMBL4750385 IC50 = 200.0 nM 6.70
CHEMBL4745746 IC50 = 200.0 nM 6.70
CHEMBL4750236 IC50 = 230.0 nM 6.64
CHEMBL4791004 IC50 = 250.0 nM 6.60
CHEMBL4800086 IC50 = 320.0 nM 6.50
CHEMBL4781485 IC50 = 440.0 nM 6.36
CHEMBL4800583 IC50 = 450.0 nM 6.35
CHEMBL4744508 IC50 = 570.0 nM 6.24