Assay Detail

CHEMBL4673755

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Binding

Inhibition of recombinant Staphylococcus aureus His-tagged DHFR using DiHF as substrate incubated for 3 mins

ChEMBL ↗

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Target

Unchecked (CHEMBL612545) ↗

Open DB target context

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Unchecked (CHEMBL612545) is the working anchor for this assay review. Loading the live Open DB target snapshot…

Primary target Unchecked

CHEMBL612545

Anchored from Assay target.

Protein identity Unchecked

Disease frame Disease evidence pending

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Review focus Open DB context pending

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Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Binding
Organism	Staphylococcus aureus
Confidence	0 — Uncurated / Unknown
Curated By	Autocuration

Target

Unchecked (CHEMBL612545) ↗

Type

UNCHECKED

Publication

Identification of P218 as a potent inhibitor of Mycobacterium ulcerans DHFR

Riboldi, Gustavo P., Zigweid, Rachael, Myler, Peter J., Mayclin, Stephen J., CouA+/-ago, Rafael M., Staker, Bart L.
RSC Med Chem (2021) 12 : 103 -109
PubMed 34046602 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
Ki	1	8.36	8.36
IC50	1	10.85	10.85

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL22	TRIMETHOPRIM	4.0	2	10.85

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL22	TRIMETHOPRIM	IC50	=	0.014	nM	10.85
CHEMBL22	TRIMETHOPRIM	Ki	=	4.4	nM	8.36