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Assay Detail

CHEMBL4676232

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Tag-lite green-labeled agonist binding to terbium fluorophore-labeled human N-terminal SNAP-tag GnRh receptor expressed in HEK293 cells assessed as equilibrium dissociation constant by TR-FRET assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Gonadotropin-releasing hormone receptor (CHEMBL1855)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Characterization of BAY 1214784, an Orally Available Spiroindoline Derivative Acting as a Potent and Selective Antagonist of the Human Gonadotropin-Releasing Hormone Receptor as Proven in a First-In-Human Study in Postmenopausal Women.
Panknin O,Wagenfeld A,Bone W,Bender E,Nowak-Reppel K,Fernández-Montalván AE,Nubbemeyer R,Bäurle S,Ring S,Schmees N,Prien O,Schäfer M,Friedrich C,Zollner TM,Steinmeyer A,Mueller T,Langer G
J Med Chem (2020) 63 : 11854 -11881
PubMed 32960053 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 4 9.03 9.87

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1200490 CETRORELIX 4.0 1 9.87
CHEMBL1800159 RELUGOLIX 4.0 1 9.08
CHEMBL502182 ELAGOLIX SODIUM 4.0 1 8.92
CHEMBL4537788 BAY-784 1 8.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1200490 CETRORELIX Kd = 0.135 nM 9.87
CHEMBL1800159 RELUGOLIX Kd = 0.84 nM 9.08
CHEMBL502182 ELAGOLIX SODIUM Kd = 1.19 nM 8.92
CHEMBL4537788 BAY-784 Kd = 5.52 nM 8.26