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Assay Detail

CHEMBL4678516

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Binding
Inhibition of human PARP-1 catalytic domain (662 to 1011 residues) expressed in Escherichia coli BL21(DE3) cells incubated for 0.5 hrs by fluorescence polarization assay based DNA trapping activity assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Poly [ADP-ribose] polymerase 1 (CHEMBL3105)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Pamiparib (BGB-290), a Potent and Selective Poly (ADP-ribose) Polymerase (PARP) Inhibitor in Clinical Development.
Wang H,Ren B,Liu Y,Jiang B,Guo Y,Wei M,Luo L,Kuang X,Qiu M,Lv L,Xu H,Qi R,Yan H,Xu D,Wang Z,Huo CX,Zhu Y,Zhao Y,Wu Y,Qin Z,Su D,Tang T,Wang F,Sun X,Feng Y,Peng H,Wang X,Gao Y,Liu Y,Gong W,Yu F,Liu X,Wang L,Zhou C
J Med Chem (2020) 63 : 15541 -15563
PubMed 33264017 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 7.64 8.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3137320 TALAZOPARIB 4.0 1 8.49
CHEMBL4112930 PAMIPARIB 3.0 1 7.89
CHEMBL1173055 RUCAPARIB 4.0 1 7.77
CHEMBL506871 VELIPARIB 3.0 1 6.40
CHEMBL521686 OLAPARIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3137320 TALAZOPARIB EC50 = 3.2 nM 8.49
CHEMBL4112930 PAMIPARIB EC50 = 13.0 nM 7.89
CHEMBL1173055 RUCAPARIB EC50 = 17.0 nM 7.77
CHEMBL506871 VELIPARIB EC50 = 400.0 nM 6.40
CHEMBL521686 OLAPARIB EC50 = 16.0 nM -