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Assay Detail

CHEMBL4679290

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-epibatidine from human alpha4beta2 nAChR S108A mutant transfected in human Hela cell membranes preincubated for 5 mins followed by [3H]-Epibatidine addition and measured after overnight incubation by liquid scintillation beta counter analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Modifications at C(5) of 2-(2-Pyrrolidinyl)-Substituted 1,4-Benzodioxane Elicit Potent α4β2 Nicotinic Acetylcholine Receptor Partial Agonism with High Selectivity over the α3β4 Subtype.
Bavo F,Pallavicini M,Gotti C,Appiani R,Moretti M,Colombo SF,Pucci S,Viani P,Budriesi R,Renzi M,Fucile S,Bolchi C
J Med Chem (2020) 63 : 15668 -15692
PubMed 33325696 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 7.31 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1917231 1 8.92
CHEMBL4785648 1 8.22
CHEMBL4741357 1 6.81
CHEMBL4095303 1 6.58
CHEMBL1186866 1 6.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1917231 Ki = 1.2 nM 8.92
CHEMBL4785648 Ki = 6.0 nM 8.22
CHEMBL4741357 Ki = 156.0 nM 6.81
CHEMBL4095303 Ki = 265.0 nM 6.58
CHEMBL1186866 Ki = 912.0 nM 6.04