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Assay Detail

CHEMBL4679740

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to BTN3A1 in human PBMC-derived Vgamma9/Vdelta2 T cells assessed as induction of Vgamma9Vdelta2 T cells proliferation incubated for 3 days by gamma delta TCR/PE-CD3+ antibody staining based FACS analysis
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Vgamma9Vdelta2 T cell
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Butyrophilin subfamily 3 member A1 (CHEMBL4105758)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and Metabolism of BTN3A1 Ligands: Studies on Modifications of the Allylic Alcohol.
Lentini NA,Schroeder CM,Harmon NM,Huang X,Schladetsch MA,Foust BJ,Poe MM,Hsiao CC,Wiemer AJ,Wiemer DF
ACS Med Chem Lett (2021) 12 : 136 -142
PubMed 33488975 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 7.38 8.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4746099 1 8.34
CHEMBL4088948 1 8.27
CHEMBL4744593 1 8.10
CHEMBL4745129 1 7.92
CHEMBL4739889 1 7.70
CHEMBL4743999 1 7.33
CHEMBL4776906 1 7.33
CHEMBL4780556 1 7.31
CHEMBL4742284 1 6.10
CHEMBL4172453 1 5.40
CHEMBL4780307 1 -
CHEMBL4746944 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4746099 EC50 = 4.6 nM 8.34
CHEMBL4088948 EC50 = 5.4 nM 8.27
CHEMBL4744593 EC50 = 8.0 nM 8.10
CHEMBL4745129 EC50 = 12.0 nM 7.92
CHEMBL4739889 EC50 = 20.0 nM 7.70
CHEMBL4743999 EC50 = 47.0 nM 7.33
CHEMBL4776906 EC50 = 47.0 nM 7.33
CHEMBL4780556 EC50 = 49.0 nM 7.31
CHEMBL4742284 EC50 = 790.0 nM 6.10
CHEMBL4172453 EC50 = 4000.0 nM 5.40
CHEMBL4780307 EC50 > 100000.0 nM -
CHEMBL4746944 EC50 > 100000.0 nM -