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Assay Detail

CHEMBL4681104

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human 5-HT2C INI receptor expressed in human HEK293 cells assessed as induction of beta-arrestin-2 recruitment incubated for 20 hrs by Tango assay
18
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2C (CHEMBL225)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Discovery of Functionally Selective Serotonin 2C (5-HT) Receptor Agonists.
Cheng J,McCorvy JD,Giguere PM,Zhu H,Kenakin T,Roth BL,Kozikowski AP
J Med Chem (2016) 59 : 9866 -9880
PubMed 27726356 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 18 7.31 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2331803 2 8.00
CHEMBL4790992 2 7.71
CHEMBL39 SEROTONIN 3.0 2 7.68
CHEMBL4779260 2 7.60
CHEMBL4781163 2 7.52
CHEMBL360328 LORCASERIN 4.0 2 7.23
CHEMBL4761064 2 7.02
CHEMBL4743977 2 6.57
CHEMBL4752976 2 6.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2331803 EC50 = 10.0 nM 8.00
CHEMBL2331803 EC50 = 10.72 nM 7.97
CHEMBL4790992 EC50 = 19.5 nM 7.71
CHEMBL4790992 EC50 = 20.0 nM 7.70
CHEMBL39 SEROTONIN EC50 = 21.0 nM 7.68
CHEMBL39 SEROTONIN EC50 = 21.88 nM 7.66
CHEMBL4779260 EC50 = 25.12 nM 7.60
CHEMBL4779260 EC50 = 25.0 nM 7.60
CHEMBL4781163 EC50 = 30.0 nM 7.52
CHEMBL4781163 EC50 = 30.2 nM 7.52
CHEMBL360328 LORCASERIN EC50 = 58.88 nM 7.23
CHEMBL360328 LORCASERIN EC50 = 59.0 nM 7.23
CHEMBL4761064 EC50 = 95.5 nM 7.02
CHEMBL4761064 EC50 = 96.0 nM 7.02
CHEMBL4743977 EC50 = 267.0 nM 6.57
CHEMBL4743977 EC50 = 269.15 nM 6.57
CHEMBL4752976 EC50 = 318.0 nM 6.50
CHEMBL4752976 EC50 = 323.59 nM 6.49