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Assay Detail

CHEMBL4681676

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity in human SK-HEP1 cells by sulforhodamine B colorimetric assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type SK-HEP1
Confidence 1 — Organism
Curated By Autocuration

Target

SK-HEP1 (CHEMBL614912)
Type CELL-LINE
Organism Homo sapiens

Publication

Discovery of Leucyladenylate Sulfamates as Novel Leucyl-tRNA Synthetase (LRS)-Targeted Mammalian Target of Rapamycin Complex 1 (mTORC1) Inhibitors.
Yoon S,Kim JH,Kim SE,Kim C,Tran PT,Ann J,Koh Y,Jang J,Kim S,Moon HS,Kim WK,Lee S,Lee J,Kim S,Lee J
J Med Chem (2016) 59 : 10322 -10328
PubMed 27933890 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.82 6.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL44657 ETOPOSIDE 4.0 1 6.60
CHEMBL1163081 LEUSA 1 6.27
CHEMBL4104169 1 5.25
CHEMBL4558130 1 5.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL44657 ETOPOSIDE IC50 = 250.0 nM 6.60
CHEMBL1163081 LEUSA IC50 = 540.0 nM 6.27
CHEMBL4104169 IC50 = 5630.0 nM 5.25
CHEMBL4558130 IC50 = 6810.0 nM 5.17