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Assay Detail

CHEMBL4682704

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at PAC1 receptor (unknown origin) expressed in CHO cells assessed as inhibition of PACAP-induced inhibition of CREB phosphorylation preincubated for 30 mins followed by PACAP stimulation and measured after 30 mins by Western blot analysis
4
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Synthesis of a novel and potent small-molecule antagonist of PAC1 receptor for the treatment of neuropathic pain.
Takasaki I,Ogashi H,Okada T,Shimodaira A,Hayakawa D,Watanabe A,Miyata A,Kurihara T,Gouda H,Toyooka N
Eur J Med Chem (2020) 186 : 111902 -111902
PubMed 31771828 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Inhibition 2 - -
IC50 2 9.74 9.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4757203 2 9.74
CHEMBL4740931 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4757203 IC50 = 0.182 nM 9.74
CHEMBL4757203 Inhibition - % -
CHEMBL4740931 Inhibition - % -
CHEMBL4740931 IC50 < 0.01 nM -