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Assay Detail

CHEMBL4685391

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Binding
Inhibition of sulfasalazine-stimulated ABCB1 ATPase activity (unknown origin) expressed in baculovirus infected Sf9 insect cells using ATP as substrate measured after 1 hr by colorimetric assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tariquidar-related triazoles as potent, selective and stable inhibitors of ABCG2 (BCRP).
Antoni F,Bause M,Scholler M,Bauer S,Stark SA,Jackson SM,Manolaridis I,Locher KP,König B,Buschauer A,Bernhardt G
Eur J Med Chem (2020) 191 : 112133 -112133
PubMed 32105979 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.55 7.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4790452 1 7.03
CHEMBL4764773 1 7.02
CHEMBL2011282 1 6.71
CHEMBL4534001 1 6.65
CHEMBL488910 1 6.57
CHEMBL502503 1 6.53
CHEMBL2011288 1 6.13
CHEMBL410316 FUMITREMORGIN 1 5.74
CHEMBL348475 TARIQUIDAR 3.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4790452 IC50 = 93.0 nM 7.03
CHEMBL4764773 IC50 = 96.0 nM 7.02
CHEMBL2011282 IC50 = 194.0 nM 6.71
CHEMBL4534001 IC50 = 226.0 nM 6.65
CHEMBL488910 IC50 = 271.0 nM 6.57
CHEMBL502503 IC50 = 294.0 nM 6.53
CHEMBL2011288 IC50 = 745.0 nM 6.13
CHEMBL410316 FUMITREMORGIN IC50 = 1808.0 nM 5.74
CHEMBL348475 TARIQUIDAR IC50 >= 1180.0 nM -