Assay Detail
Binding
CHEMBL4685848
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human full-length N-terminal GST-tagged HDAC6 expressed in baculovirus infected Sf9 insect cells using Boc-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Synthesis and biological evaluation of acridine-based histone deacetylase inhibitors as multitarget agents against Alzheimer's disease.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 13 | 6.88 | 7.60 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4762873 | — | — | 1 | 7.60 |
| CHEMBL4796066 | — | — | 1 | 7.58 |
| CHEMBL4757035 | — | — | 1 | 7.54 |
| CHEMBL4751082 | — | — | 1 | 7.54 |
| CHEMBL4752558 | — | — | 1 | 7.41 |
| CHEMBL4757768 | — | — | 1 | 7.13 |
| CHEMBL98 | VORINOSTAT | 4.0 | 1 | 7.08 |
| CHEMBL4248676 | — | — | 1 | 6.85 |
| CHEMBL4753795 | — | — | 1 | 6.80 |
| CHEMBL4761847 | — | — | 1 | 6.66 |
| CHEMBL4750838 | — | — | 1 | 6.47 |
| CHEMBL4799871 | — | — | 1 | 5.45 |
| CHEMBL4753828 | — | — | 1 | 5.33 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4762873 | — | IC50 | = | 25.0 | nM | 7.60 |
| CHEMBL4796066 | — | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL4757035 | — | IC50 | = | 29.0 | nM | 7.54 |
| CHEMBL4751082 | — | IC50 | = | 29.0 | nM | 7.54 |
| CHEMBL4752558 | — | IC50 | = | 39.0 | nM | 7.41 |
| CHEMBL4757768 | — | IC50 | = | 74.0 | nM | 7.13 |
| CHEMBL98 | VORINOSTAT | IC50 | = | 83.0 | nM | 7.08 |
| CHEMBL4248676 | — | IC50 | = | 140.0 | nM | 6.85 |
| CHEMBL4753795 | — | IC50 | = | 160.0 | nM | 6.80 |
| CHEMBL4761847 | — | IC50 | = | 220.0 | nM | 6.66 |
| CHEMBL4750838 | — | IC50 | = | 340.0 | nM | 6.47 |
| CHEMBL4799871 | — | IC50 | = | 3550.0 | nM | 5.45 |
| CHEMBL4753828 | — | IC50 | = | 4690.0 | nM | 5.33 |