Assay Detail

CHEMBL4687619

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Binding

Inhibition of human CD73 (1 to 547 residues) expressed in HEK293F cells using 0.023 uM AMP as substrate by rapidfire mass spectrometry assay

ChEMBL ↗

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Target

5'-nucleotidase (CHEMBL5957) ↗

Open DB target context

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Primary target 5'-nucleotidase

CHEMBL5957

Anchored from Assay target.

Protein identity 5'-nucleotidase

Disease frame Disease evidence pending

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Review focus Open DB context pending

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Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Binding
Organism	Homo sapiens
Cell Type	HEK293F
Confidence	9 — Direct single protein target
Curated By	Autocuration

Target

5'-nucleotidase (CHEMBL5957) ↗

Type	SINGLE PROTEIN
Organism	Homo sapiens

Publication

Targeting Metabolism of Extracellular Nucleotides via Inhibition of Ectonucleotidases CD73 and CD39.

Jeffrey JL,Lawson KV,Powers JP
J Med Chem (2020) 63 : 13444 -13465
PubMed 32786396 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
IC50	1	6.39	6.39

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL4792487	LY-3475070	1.0	1	6.39

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL4792487	LY-3475070	IC50	=	409.0	nM	6.39