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Assay Detail

CHEMBL4687755

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at TLR2 in HEK-Blue hTLR2 cells assessed as NF-kappaB activation preincubated for 24 hrs followed by replacement of medium containing compound and measured after 0.5 to 1 hrs by Quanti-blue SEAP assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK-Blue hTLR2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Toll-like receptor 2 (CHEMBL4163)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Modulation of the Innate Immune Response by Targeting Toll-like Receptors: A Perspective on Their Agonists and Antagonists.
Federico S,Pozzetti L,Papa A,Carullo G,Gemma S,Butini S,Campiani G,Relitti N
J Med Chem (2020) 63 : 13466 -13513
PubMed 32845153 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 2 7.29 8.31

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4759149 1 8.31
CHEMBL4581678 1 6.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4759149 EC50 = 4.88 nM 8.31
CHEMBL4581678 EC50 = 550.0 nM 6.26