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Assay Detail

CHEMBL4688567

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal His6-tagged GSK3beta H350L mutant expressed in baculovirus infected Sf21 cells using prephosphorylated polypeptide as substrate incubated for 1 min followed by substrate addition and measured after 30 mins by Kinase-Glo luminescence assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glycogen synthase kinase-3 beta (CHEMBL262)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent glycogen synthase kinase 3/cholinesterase inhibitors with neuroprotection as potential therapeutic agent for Alzheimer's disease.
Jiang X,Wang Y,Liu C,Xing C,Wang Y,Lyu W,Wang S,Li Q,Chen T,Chen Y,Feng F,Liu W,Sun H
Bioorg Med Chem (2021) 30 : 115940 -115940
PubMed 33340937 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 7.67 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4747992 1 8.52
CHEMBL4792678 1 8.30
CHEMBL4755445 1 8.30
CHEMBL4752296 1 8.15
CHEMBL4794823 1 8.10
CHEMBL4776811 1 8.05
CHEMBL4756451 1 8.00
CHEMBL4787873 1 7.96
CHEMBL4753139 1 7.85
CHEMBL4748993 1 7.82
CHEMBL4748792 1 7.80
CHEMBL4796437 1 7.72
CHEMBL102714 SB-216763 1 7.70
CHEMBL4795876 1 7.58
CHEMBL4792741 1 7.58
CHEMBL4742138 1 7.57
CHEMBL4764558 1 7.42
CHEMBL4746460 1 7.39
CHEMBL4791089 1 7.35
CHEMBL4785551 1 7.31
CHEMBL4741669 1 7.21
CHEMBL4753478 1 7.03
CHEMBL4749550 1 6.71
CHEMBL4759253 1 6.58
CHEMBL502 DONEPEZIL 4.0 1 -
CHEMBL95 TACRINE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4747992 IC50 = 3.0 nM 8.52
CHEMBL4792678 IC50 = 5.0 nM 8.30
CHEMBL4755445 IC50 = 5.0 nM 8.30
CHEMBL4752296 IC50 = 7.0 nM 8.15
CHEMBL4794823 IC50 = 8.0 nM 8.10
CHEMBL4776811 IC50 = 9.0 nM 8.05
CHEMBL4756451 IC50 = 10.0 nM 8.00
CHEMBL4787873 IC50 = 11.0 nM 7.96
CHEMBL4753139 IC50 = 14.0 nM 7.85
CHEMBL4748993 IC50 = 15.0 nM 7.82
CHEMBL4748792 IC50 = 16.0 nM 7.80
CHEMBL4796437 IC50 = 19.0 nM 7.72
CHEMBL102714 SB-216763 IC50 = 20.0 nM 7.70
CHEMBL4795876 IC50 = 26.0 nM 7.58
CHEMBL4792741 IC50 = 26.0 nM 7.58
CHEMBL4742138 IC50 = 27.0 nM 7.57
CHEMBL4764558 IC50 = 38.0 nM 7.42
CHEMBL4746460 IC50 = 41.0 nM 7.39
CHEMBL4791089 IC50 = 45.0 nM 7.35
CHEMBL4785551 IC50 = 49.0 nM 7.31
CHEMBL4741669 IC50 = 62.0 nM 7.21
CHEMBL4753478 IC50 = 94.0 nM 7.03
CHEMBL4749550 IC50 = 193.0 nM 6.71
CHEMBL4759253 IC50 = 261.0 nM 6.58
CHEMBL502 DONEPEZIL IC50 > 1000.0 nM -
CHEMBL95 TACRINE IC50 > 1000.0 nM -