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Assay Detail

CHEMBL4689490

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal GST-tagged HPK1 (1 to 346 residues) expressed in baculovirus infected Sf9 cells using NH2-fluorescein-RFARKGSLRQKNV-COOH as substrate incubated for 3 hrs in presence of ATP by by capillary electrophoresis method
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of Orally Active Isofuranones as Potent, Selective Inhibitors of Hematopoetic Progenitor Kinase 1.
Degnan AP,Kumi GK,Allard CW,Araujo EV,Johnson WL,Zimmermann K,Pearce BC,Sheriff S,Futran A,Li X,Locke GA,You D,Morrison J,Parrish KE,Stromko C,Murtaza A,Liu J,Johnson BM,Vite GD,Wittman MD
ACS Med Chem Lett (2021) 12 : 443 -450
PubMed 33732413 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 8.23 9.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4778959 1 9.66
CHEMBL4780798 1 9.55
CHEMBL4789200 1 8.92
CHEMBL4750822 1 8.89
CHEMBL4759406 1 8.85
CHEMBL4758114 1 8.82
CHEMBL4779496 1 8.66
CHEMBL4748154 1 8.59
CHEMBL4784181 1 8.59
CHEMBL4790192 1 8.40
CHEMBL4784784 1 8.40
CHEMBL4761884 1 8.29
CHEMBL4748045 1 8.14
CHEMBL4749598 1 8.03
CHEMBL4741772 1 8.00
CHEMBL4745378 1 7.85
CHEMBL4776227 1 7.82
CHEMBL4758887 1 7.80
CHEMBL4793633 1 7.70
CHEMBL4758572 1 7.58
CHEMBL4761304 1 7.54
CHEMBL4793479 1 6.96
CHEMBL4752693 1 6.14
CHEMBL4792398 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4778959 IC50 = 0.22 nM 9.66
CHEMBL4780798 IC50 = 0.28 nM 9.55
CHEMBL4789200 IC50 = 1.2 nM 8.92
CHEMBL4750822 IC50 = 1.3 nM 8.89
CHEMBL4759406 IC50 = 1.4 nM 8.85
CHEMBL4758114 IC50 = 1.5 nM 8.82
CHEMBL4779496 IC50 = 2.2 nM 8.66
CHEMBL4748154 IC50 = 2.6 nM 8.59
CHEMBL4784181 IC50 = 2.6 nM 8.59
CHEMBL4790192 IC50 = 4.0 nM 8.40
CHEMBL4784784 IC50 = 4.0 nM 8.40
CHEMBL4761884 IC50 = 5.1 nM 8.29
CHEMBL4748045 IC50 = 7.3 nM 8.14
CHEMBL4749598 IC50 = 9.3 nM 8.03
CHEMBL4741772 IC50 = 10.0 nM 8.00
CHEMBL4745378 IC50 = 14.0 nM 7.85
CHEMBL4776227 IC50 = 15.0 nM 7.82
CHEMBL4758887 IC50 = 16.0 nM 7.80
CHEMBL4793633 IC50 = 20.0 nM 7.70
CHEMBL4758572 IC50 = 26.0 nM 7.58
CHEMBL4761304 IC50 = 29.0 nM 7.54
CHEMBL4793479 IC50 = 110.0 nM 6.96
CHEMBL4752693 IC50 = 730.0 nM 6.14
CHEMBL4792398 IC50 > 2000.0 nM -