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Assay Detail

CHEMBL4689582

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at recombinant human A2BAR expressed in CHO cells assessed as stimulation of cAMP formation preincubated for 30 mins in presence of rolipram and adenosine deaminase followed by compound addition and measured after 20 mins by alphascreen cAMP assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2b (CHEMBL255)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Biological Evaluation of 5'-(N-Ethylcarboxamido)adenosine Analogues as Grp94-Selective Inhibitors.
Tosh DK,Brackett CM,Jung YH,Gao ZG,Banerjee M,Blagg BSJ,Jacobson KA
ACS Med Chem Lett (2021) 12 : 373 -379
PubMed 33738064 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 2 6.67 7.02

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL464859 NECA 1 7.02
CHEMBL193692 1 6.31

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL464859 NECA EC50 = 94.8 nM 7.02
CHEMBL193692 EC50 = 485.0 nM 6.31