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Assay Detail

CHEMBL4689686

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of IDO1 in LPS and IFN-gamma-stimulated human Whole blood assessed as reduction in kynurenine production using isotope-labeled tryptophan as substrate incubated for 15 mins followed by LPS and IFN-gamma stimulation measured after 18 hrs by LC-MS/MS analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Indoleamine 2,3-dioxygenase 1 (CHEMBL4685)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Carbamate and N-Pyrimidine Mitigate Amide Hydrolysis: Structure-Based Drug Design of Tetrahydroquinoline IDO1 Inhibitors.
Li D,Deng Y,Achab A,Bharathan I,Hopkins BA,Yu W,Zhang H,Sanyal S,Pu Q,Zhou H,Liu K,Lim J,Fradera X,Lesburg CA,Lammens A,Martinot TA,Cohen RD,Doty AC,Ferguson H,Nickbarg EB,Cheng M,Spacciapoli P,Geda P,Song X,Smotrov N,Abeywickrema P,Andrews C,Chamberlin C,Mabrouk O,Curran P,Richards M,Saradjian P,Miller JR,Knemeyer I,Otte KM,Vincent S,Sciammetta N,Pasternak A,Bennett DJ,Han Y
ACS Med Chem Lett (2021) 12 : 389 -396
PubMed 33738066 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.84 7.58

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4744727 1 7.58
CHEMBL4783395 1 7.38
CHEMBL4749009 1 6.90
CHEMBL4788767 1 5.49

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4744727 IC50 = 26.0 nM 7.58
CHEMBL4783395 IC50 = 42.0 nM 7.38
CHEMBL4749009 IC50 = 127.0 nM 6.90
CHEMBL4788767 IC50 = 3249.0 nM 5.49