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Assay Detail

CHEMBL4699703

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human MAO- A using p-tyramine as substrate preincubated with enzyme for 15 mins followed incubation with substrate for 20 mins by Amplex red reagent based fluorescence based analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Amine oxidase [flavin-containing] A (CHEMBL1951)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Chromone and donepezil hybrids as new multipotent cholinesterase and monoamine oxidase inhibitors for the potential treatment of Alzheimer''s disease
Wang, Xiao-Bing, Yin, Fu-Cheng, Huang, Ming, Jiang, Neng, Lan, Jin-Shuai, Kong, Ling-Yi
RSC Med Chem (2020) 11 : 225 -233
PubMed 33479629 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 4.78 5.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL92401 IPRONIAZID 2.0 1 5.23
CHEMBL4760340 1 4.94
CHEMBL4762982 1 4.17
CHEMBL502 DONEPEZIL 4.0 1 -
CHEMBL673 PARGYLINE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL92401 IPRONIAZID IC50 = 5890.0 nM 5.23
CHEMBL4760340 IC50 = 11600.0 nM 4.94
CHEMBL4762982 IC50 = 67200.0 nM 4.17
CHEMBL502 DONEPEZIL IC50 - -
CHEMBL673 PARGYLINE IC50 - -