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Assay Detail

CHEMBL4701416

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of His-tagged human CDK2/cyclin E1 expressed in insect cells using biotin Ttds-YISPLKSPYKISEG as substrate preincubated for 15 mins followed by ATP and substrate addition and measured after 25 mins by TR-FRET assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Cyclin-dependent kinase 2/cyclin E1 (CHEMBL1907605)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Recent Developments in the Biology and Medicinal Chemistry of CDK9 Inhibitors: An Update.
Wu T,Qin Z,Tian Y,Wang J,Xu C,Li Z,Bian J
J Med Chem (2020) 63 : 13228 -13257
PubMed 32866383 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.06 6.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4789982 1 6.25
CHEMBL4756226 1 6.22
CHEMBL4743691 1 5.97
CHEMBL4742789 1 5.79

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4789982 IC50 = 558.0 nM 6.25
CHEMBL4756226 IC50 = 608.0 nM 6.22
CHEMBL4743691 IC50 = 1078.0 nM 5.97
CHEMBL4742789 IC50 = 1608.0 nM 5.79