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Assay Detail

CHEMBL4705014

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of C-terminal hexahistidine tag in human recombinant PNMT expressed in Escherichia coli assessed as inhibition constant using PEA as substrate in presence of 100 uM AdoMet as co-substrate by Sigma-plot analysis
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Phenylethanolamine N-methyltransferase (CHEMBL4617)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Drug Design of Bisubstrate Inhibitors of Phenylethanolamine N-Methyltransferase Possessing Low Nanomolar Affinity at Both Substrate Binding Domains.
Lu J,Bart AG,Wu Q,Criscione KR,McLeish MJ,Scott EE,Grunewald GL
J Med Chem (2020) 63 : 13878 -13898
PubMed 33147410 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 21 7.12 8.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4764318 1 8.68
CHEMBL4751862 1 8.49
CHEMBL4782649 1 8.48
CHEMBL4760727 1 8.46
CHEMBL4800292 1 8.42
CHEMBL4754656 1 8.41
CHEMBL4749263 1 7.75
CHEMBL4740168 1 7.42
CHEMBL4795252 1 7.34
CHEMBL4796664 1 7.24
CHEMBL4789060 1 7.16
CHEMBL4791266 1 7.15
CHEMBL4791373 1 7.07
CHEMBL4787632 1 6.09
CHEMBL4754362 1 5.37
CHEMBL4783068 1 5.29
CHEMBL418052 S-ADENOSYLHOMOCYSTEINE 1 4.85
CHEMBL563938 1 4.55
CHEMBL59441 1 -
CHEMBL60724 1 -
CHEMBL4749860 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4764318 Ki = 2.1 nM 8.68
CHEMBL4751862 Ki = 3.2 nM 8.49
CHEMBL4782649 Ki = 3.3 nM 8.48
CHEMBL4760727 Ki = 3.5 nM 8.46
CHEMBL4800292 Ki = 3.8 nM 8.42
CHEMBL4754656 Ki = 3.9 nM 8.41
CHEMBL4749263 Ki = 18.0 nM 7.75
CHEMBL4740168 Ki = 38.0 nM 7.42
CHEMBL4795252 Ki = 46.0 nM 7.34
CHEMBL4796664 Ki = 57.0 nM 7.24
CHEMBL4789060 Ki = 70.0 nM 7.16
CHEMBL4791266 Ki = 71.0 nM 7.15
CHEMBL4791373 Ki = 86.0 nM 7.07
CHEMBL4787632 Ki = 810.0 nM 6.09
CHEMBL4754362 Ki = 4300.0 nM 5.37
CHEMBL4783068 Ki = 5100.0 nM 5.29
CHEMBL418052 S-ADENOSYLHOMOCYSTEINE Ki = 14000.0 nM 4.85
CHEMBL563938 Ki = 28000.0 nM 4.55
CHEMBL59441 Ki - -
CHEMBL60724 Ki - -
CHEMBL4749860 Ki - -