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Assay Detail

CHEMBL4709752

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant GST-tagged human CDK2/GST-tagged cyclin E expressed in sf9 cells using biotinylated peptide biotin-Ttds-YISPLKSPYKISEG as substrate preincubated for 15 mins followed by ATP and substrate addition and measured after 25 mins by TR-FRET assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Cyclin-dependent kinase 2/cyclin E1 (CHEMBL1907605)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Sulfoximines as Rising Stars in Modern Drug Discovery? Current Status and Perspective on an Emerging Functional Group in Medicinal Chemistry.
Mäder P,Kattner L
J Med Chem (2020) 63 : 14243 -14275
PubMed 32870008 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.57 5.97

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4743691 1 5.97
CHEMBL4439321 ATUVECICLIB 1.0 1 5.96
CHEMBL4762845 1 5.54
CHEMBL3962241 1 5.40
CHEMBL4793497 1 4.98

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4743691 IC50 = 1078.0 nM 5.97
CHEMBL4439321 ATUVECICLIB IC50 = 1100.0 nM 5.96
CHEMBL4762845 IC50 = 2920.0 nM 5.54
CHEMBL3962241 IC50 = 3959.0 nM 5.40
CHEMBL4793497 IC50 = 10504.0 nM 4.98