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Assay Detail

CHEMBL4711026

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-N-methylspiperone from human D2L receptor expressed in HEK293 cell membranes measured after 60 mins by microbeta scintillation counting method
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(2) dopamine receptor (CHEMBL217)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationships for a series of (Bis(4-fluorophenyl)methyl)sulfinylethyl-aminopiperidines and -piperidine amines at the dopamine transporter: Bioisosteric replacement of the piperazine improves metabolic stability.
Giancola JB,Bonifazi A,Cao J,Ku T,Haraczy AJ,Lam J,Rais R,Coggiano MA,Tanda G,Newman AH
Eur J Med Chem (2020) 208 : 112674 -112674
PubMed 32947229 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 5.56 7.11

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3918282 1 7.11
CHEMBL3931765 1 6.53
CHEMBL3916231 1 6.16
CHEMBL4798638 1 5.64
CHEMBL4794406 1 5.54
CHEMBL4744824 1 5.54
CHEMBL4750607 1 5.40
CHEMBL4765081 1 5.22
CHEMBL4758774 1 5.18
CHEMBL4791063 1 5.13
CHEMBL4741279 1 5.05
CHEMBL4636778 1 4.92
CHEMBL4750660 1 4.91
CHEMBL1373 MODAFINIL 4.0 1 -
CHEMBL4777080 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3918282 Ki = 78.6 nM 7.11
CHEMBL3931765 Ki = 298.0 nM 6.53
CHEMBL3916231 Ki = 693.0 nM 6.16
CHEMBL4798638 Ki = 2270.0 nM 5.64
CHEMBL4794406 Ki = 2920.0 nM 5.54
CHEMBL4744824 Ki = 2900.0 nM 5.54
CHEMBL4750607 Ki = 4020.0 nM 5.40
CHEMBL4765081 Ki = 6070.0 nM 5.22
CHEMBL4758774 Ki = 6610.0 nM 5.18
CHEMBL4791063 Ki = 7440.0 nM 5.13
CHEMBL4741279 Ki = 8830.0 nM 5.05
CHEMBL4636778 Ki = 11900.0 nM 4.92
CHEMBL4750660 Ki = 12400.0 nM 4.91
CHEMBL1373 MODAFINIL Ki > 100000.0 nM -
CHEMBL4777080 Ki = 156000.0 nM -