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Assay Detail

CHEMBL4711086

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal His6-tagged GSK-3beta H350L mutant expressed in baculovirus infected Sf21 cells using GSM as substrate incubated for 30 mins in presence of ATP by kinase-glo luminescence assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glycogen synthase kinase-3 beta (CHEMBL262)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rational design and biological evaluation of a new class of thiazolopyridyl tetrahydroacridines as cholinesterase and GSK-3 dual inhibitors for Alzheimer's disease.
Jiang X,Zhou J,Wang Y,Chen L,Duan Y,Huang J,Liu C,Chen Y,Liu W,Sun H,Feng F,Qu W
Eur J Med Chem (2020) 207 : 112751 -112751
PubMed 32950908 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 7.30 8.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4747410 1 8.19
CHEMBL4744911 1 7.84
CHEMBL3410089 1 7.76
CHEMBL102714 SB-216763 1 7.70
CHEMBL4779478 1 7.68
CHEMBL4789145 1 7.66
CHEMBL4756424 1 7.58
CHEMBL4799661 1 7.51
CHEMBL4787590 1 7.43
CHEMBL4785004 1 7.41
CHEMBL4749327 1 7.39
CHEMBL4756997 1 7.38
CHEMBL4753347 1 7.37
CHEMBL4741219 1 7.33
CHEMBL4791286 1 7.29
CHEMBL4798419 1 7.21
CHEMBL4800403 1 7.21
CHEMBL4777788 1 7.20
CHEMBL4760288 1 7.19
CHEMBL4787169 1 7.14
CHEMBL4796602 1 7.14
CHEMBL4754192 1 7.09
CHEMBL4777926 1 7.08
CHEMBL4758531 1 7.03
CHEMBL4781117 1 6.99
CHEMBL4761239 1 6.94
CHEMBL4749678 1 6.88
CHEMBL4750284 1 6.88
CHEMBL4759230 1 6.78
CHEMBL4746403 1 6.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4747410 IC50 = 6.5 nM 8.19
CHEMBL4744911 IC50 = 14.6 nM 7.84
CHEMBL3410089 IC50 = 17.2 nM 7.76
CHEMBL102714 SB-216763 IC50 = 19.8 nM 7.70
CHEMBL4779478 IC50 = 20.8 nM 7.68
CHEMBL4789145 IC50 = 22.0 nM 7.66
CHEMBL4756424 IC50 = 26.3 nM 7.58
CHEMBL4799661 IC50 = 31.0 nM 7.51
CHEMBL4787590 IC50 = 37.1 nM 7.43
CHEMBL4785004 IC50 = 39.3 nM 7.41
CHEMBL4749327 IC50 = 41.1 nM 7.39
CHEMBL4756997 IC50 = 41.6 nM 7.38
CHEMBL4753347 IC50 = 42.6 nM 7.37
CHEMBL4741219 IC50 = 46.7 nM 7.33
CHEMBL4791286 IC50 = 51.7 nM 7.29
CHEMBL4798419 IC50 = 62.2 nM 7.21
CHEMBL4800403 IC50 = 62.0 nM 7.21
CHEMBL4777788 IC50 = 62.6 nM 7.20
CHEMBL4760288 IC50 = 64.3 nM 7.19
CHEMBL4787169 IC50 = 71.6 nM 7.14
CHEMBL4796602 IC50 = 72.0 nM 7.14
CHEMBL4754192 IC50 = 80.7 nM 7.09
CHEMBL4777926 IC50 = 83.9 nM 7.08
CHEMBL4758531 IC50 = 92.8 nM 7.03
CHEMBL4781117 IC50 = 103.6 nM 6.99
CHEMBL4761239 IC50 = 114.4 nM 6.94
CHEMBL4749678 IC50 = 130.4 nM 6.88
CHEMBL4750284 IC50 = 132.4 nM 6.88
CHEMBL4759230 IC50 = 165.3 nM 6.78
CHEMBL4746403 IC50 = 239.3 nM 6.62
CHEMBL95 TACRINE IC50 > 1000.0 nM -