Assay Detail
Binding
CHEMBL4712508
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of oligonucleotide [32P]-labelled 5'-AGCITCATTTCCCGTAAATCCCTA probe binding to STAT1/STAT3 heterodimer in mouse NIH3T3 nuclear extract preincubated for 30 mins followed by hSIE probe addition by EMSA analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | NIH3T3 |
| Subcellular | Nucleus |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
Signal transducer and activator of transcription 1/ 3 (CHEMBL3885638) ↗| Type | PROTEIN COMPLEX |
| Organism | Mus musculus |
Publication
Discovery of Novel Azetidine Amides as Potent Small-Molecule STAT3 Inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 5.51 | 5.84 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4778873 | — | — | 1 | 5.84 |
| CHEMBL4746031 | — | — | 1 | 5.58 |
| CHEMBL4753131 | — | — | 1 | 5.50 |
| CHEMBL4787896 | — | — | 1 | 5.33 |
| CHEMBL4760561 | — | — | 1 | 5.31 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4778873 | — | IC50 | = | 1460.0 | nM | 5.84 |
| CHEMBL4746031 | — | IC50 | = | 2610.0 | nM | 5.58 |
| CHEMBL4753131 | — | IC50 | = | 3140.0 | nM | 5.50 |
| CHEMBL4787896 | — | IC50 | = | 4710.0 | nM | 5.33 |
| CHEMBL4760561 | — | IC50 | = | 4920.0 | nM | 5.31 |