Assay Detail
Binding
CHEMBL4712751
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of full length mouse NAPE-PLD expressed in HEK293T cell lysate using PED6 as substrate preincubated for 30 mins followed by substrate addition and measured at 2 mins interval for 1 hr by tecan-plate reader analysis
11
Total Activities
11
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | HEK293T |
| Subcellular | Lysate |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (CHEMBL2163172) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
Structure-Activity Relationship Studies of Pyrimidine-4-Carboxamides as Inhibitors of N-Acylphosphatidylethanolamine Phospholipase D.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 5.68 | 6.42 |
| pIC50 | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4761882 | — | — | 1 | 6.42 |
| CHEMBL4782800 | — | — | 1 | 6.41 |
| CHEMBL4794048 | — | — | 1 | 6.35 |
| CHEMBL4754918 | — | — | 1 | 6.24 |
| CHEMBL4750592 | — | — | 1 | 5.90 |
| CHEMBL4760293 | — | — | 1 | 5.74 |
| CHEMBL4790197 | — | — | 1 | 5.48 |
| CHEMBL4780517 | — | — | 1 | 5.02 |
| CHEMBL496 | HEXACHLOROPHENE | 4.0 | 1 | 4.96 |
| CHEMBL4746648 | — | — | 1 | 4.27 |
| CHEMBL4750134 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4761882 | — | IC50 | = | 380.19 | nM | 6.42 |
| CHEMBL4782800 | — | IC50 | = | 389.05 | nM | 6.41 |
| CHEMBL4794048 | — | IC50 | = | 446.68 | nM | 6.35 |
| CHEMBL4754918 | — | IC50 | = | 575.44 | nM | 6.24 |
| CHEMBL4750592 | — | IC50 | = | 1258.93 | nM | 5.90 |
| CHEMBL4760293 | — | IC50 | = | 1819.7 | nM | 5.74 |
| CHEMBL4790197 | — | IC50 | = | 3311.31 | nM | 5.48 |
| CHEMBL4780517 | — | IC50 | = | 9549.93 | nM | 5.02 |
| CHEMBL496 | HEXACHLOROPHENE | IC50 | = | 11000.0 | nM | 4.96 |
| CHEMBL4746648 | — | IC50 | = | 54000.0 | nM | 4.27 |
| CHEMBL4750134 | — | pIC50 | - | — | - |